Autologous Nucleus Pulposus Attracts Monocytes In Vivo In A Pig Model. Implications For The Pathophysiology Of Sciatica
نویسندگان
چکیده
Previous experimental studies have indicated that that an autoimmune reaction towards herniated nucleus pulposus may constitute a pathogenetic component in the pathophysiology of sciatica. If an autoimmune reaction would be present, one would expect that exposure of nucleus pulposus to the host would induce an up regulation of antigen-presenting cells, such as monocytes. Autologous nucleus pulposus was placed in perforated titanium chambers, which were placed subcutaneously in pigs, together with an equal amount of empty chambers for control. After 7 days, the chambers were harvested and the number of monocytes in the chambers was determined using flow cytometry. The proportion of monocytes was significantly higher in the nucleus pulposus filled chambers than in the empty chambers. In conclusion, nucleus pulposus was shown to attract monocytes, which may suggest that monocytes might play a role in the pathophysiology of sciatica.
منابع مشابه
Combination of Two Cytokine Inhibitors Reduces Nucleus Pulposus-Induced Nerve Injury More Than Using Each Inhibitor Separately
Although recent experimental studies indicate that disc-derived cytokines, as for instance TNF, seems to be intimately involved in the pathophysiology of sciatica and low back pain, the clinical studies performed do not provide conclusive data on TNF-inhibition as a useful complement for treatment of such conditions to existing modalities. Based on the fact that TNF is merely one component in a...
متن کاملPrimary immune system responders to nucleus pulposus cells: evidence for immune response in disc herniation.
Although intervertebral disc herniation and associated sciatica is a common disease, its molecular pathogenesis is not well understood. Immune responses are thought to be involved. This study provides direct evidence that even non-degenerated nucleus pulposus (NP) cells elicit immune responses. An in vitro colony forming inhibition assay demonstrated the suppressive effects of autologous spleen...
متن کاملPathophysiology of disk-related sciatica. I.--Evidence supporting a chemical component.
Sciatica in patients with disk disease was long ascribed to pressure put on the sciatic nerve root by a herniated disk. However, a role for chemical factors acting in conjunction with this mechanical insult is suggested by a number of clinical observations: disk surgery does not consistently provide pain relief, large disk herniations are not always symptomatic, severe pain may be present in pa...
متن کاملFormation Of Nucleus Pulposus-Induced Disc Hernia-Like Nodules On The Disc Surface Is Not Induced By TNF
It was recently seen that puncture of an intervertebral disc in the rat induced disc hernia-like nodules over the puncture site and that the presence of nucleus pulposus was necessary to produce such nodules. Since TNF from the nucleus pulposus have been found to be involved in pain production the aim was to see if also TNF was involved in the formation of such nodules. Two TNF-inhibitors, infl...
متن کاملIntervertebral Disc Herniation: Pathophysiology and Emerging Therapies
Approximately 2.6 % of the US population visits a physician for treatment of spinal disorders annually (Fraser 2009 ) with costs of $7.1 billion from lost work days alone (Ricci et al. 2006 ) . “Herniation” of the intervertebral disc is one of the several spinal disorders that contribute to this very high incidence, with potential to cause signi fi cant pain, neurological de fi cit, and functio...
متن کامل